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KMID : 1145720180060020135
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Korean Herbal Medicine Informatics
2018 Volume.6 No. 2 p.135 ~ p.144
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A study on propagation of the Trichosnathis Radix (Trichosanthes kirilowii Maxim. Root) using tissue culture techniques
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Joo Su-Ji
Kim Yong-Goo
Kang Young-Min
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Abstract
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The genus Trichosanthes (Cucurbitaceae) is a perennial vine plant native to China, Japan, Mongolia and Taiwan. However, the species T. kirilowii Maxim. and T. kirilowii var. japonica Kitam. are native plants mostly in Korea and Japan. T. kirilowii, is used as a Korean and Chinese medicinal herb. However, the roots which are used in traditional medicine take several years to mature when cultivated in a traditional way. Fortunately, through tissue culture techniques, the roots can be produced in a shorter period of time. This in vitro study therefore was conducted to identify and establish the optimal medium and plant growth regulator type and concentrations suitable for adventitious root formation and biomass enhancement of T. kirilowii roots. The wild roots were collected, disinfected and cultured in MS and DJ solid and liquid agitator culture basal media supplemented with auxin-based hormone IBA to induce adventitious roots and increase the biomass within a minimal time frame. We expect to establish a system of root cultivation of T. kirilowii for the stable supply of substantial mass production in the future by providing a standard in vitro propagation protocol for the root cultivation.
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KEYWORD
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Trichosanthes kirilowii Maxim., Trichosanthes kirilowii var. japonica Kitam., adventitious root, plant growth regulators, tissue culture, in vitro propagation protocol
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